If $\Gamma : \mathbb R^n \to \mathbb R^n$ is an isometry, define
$$(U_\Gamma\psi)(x) := \psi(\Gamma^{-1}x)\quad \forall \psi \in L^2(\mathbb R^n, dx)\:.$$
With this definition you have, using the fact that the Lebesgue measure is $\Gamma$-invariant, $d\Gamma x = dx$ (which is the same as $dx = d\Gamma^{-1}x$)
$$\langle U_\Gamma \psi | U_\Gamma \phi \rangle = \int_{\mathbb R^n} \overline{\psi(\Gamma^{-1}x)} \phi(\Gamma^{-1}x) dx = \int_{\mathbb R^n} \overline{\psi(\Gamma^{-1}x)} \phi(\Gamma^{-1}x) d\Gamma^{-1} x = \int_{\mathbb R^n} \overline{\psi(x)} \phi(x) dx = \langle \psi|\phi \rangle\:.$$
This result simultaneously proves that $U_\Gamma (L^2(\mathbb R^n, dx)) \subset L^2(\mathbb R^n, dx)$ just taking $\psi=\phi \in L^2(\mathbb R^n, dx)$, and that $U_\Gamma$ preserves the scalar product of $L^2(\mathbb R^n, dx)$. To conclude that $U_\Gamma$ is unitary, it is enough to establish that it is surjective. Surjectivity is an immediate consequence of $U_\Gamma U_{\Gamma^{-1}}= U_{\Gamma \circ \Gamma^{-1}}= I$.