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Why do fluorescent beads need to be sub resolution to measure the Point Spread Functionpoint spread function?
It is mentioned everywhere that fluorescent beads need to be sub resolution for empirically measure the PSFpoint spread function (PSF). But whyWhy is this the case?
Why do fluorescent beads need to be sub resolution to measure the Point Spread Function?
It is mentioned everywhere that fluorescent beads need to be sub resolution for empirically measure the PSF. But why is this the case?
Why do fluorescent beads need to be sub resolution to measure the point spread function?
It is mentioned everywhere that fluorescent beads need to be sub resolution for empirically measure the point spread function (PSF). Why is this the case?
