Stack Exchange Network

Stack Exchange network consists of 175 Q&A communities including Stack Overflow, the largest, most trusted online community for developers to learn, share their knowledge, and build their careers.

Visit Stack Exchange

Questions tagged [microscopy]

The tag has no usage guidance.

0
votes
0answers
8 views

Question about the integrity of a TEM analysis result

I have a question concerning the integrity of a particular TEM analysis that was done. The specimen was household dust that may or may not have contained asbestos, and was sampled with Ghost Wipes, ...
0
votes
0answers
112 views

contrast in confocal microscopy

I observed fixed cells from mice tissue, thanks to confocal microscopy monochromatic 488nm. (without fluorescence staining) In some slices, cells appear in black, and in other slices in white (...
0
votes
0answers
5 views

Calculate thickness of curved membrane from confocal slices

I am imaging a thin, curvy, biological membrane using confocal microscopy. I would like to estimate the thickness of the membrane (assuming it is constant throughout). At every z-slice, I can see the ...
0
votes
1answer
32 views

How wave functions overlap in STM

In STM(Scanning Tunnelling Microscopy), as distance between tip and sample decrease tunnelling current increases. I know that when it cross barrier wave function decay exponentially. Measured current ...
0
votes
1answer
47 views

Should the working distance of an infinity-corrected objective always be smaller than the focal length?

I'm building a homebrew imaging system with a 5X infinity-corrected apochromatic objective with the following relevant specs: Working distance: $W = 45\,\mathrm{mm}$ Magnification: $M = 5\times$ ...
0
votes
1answer
56 views

Can telescope have dual purpose? [duplicate]

Astronomical telescope has a small eye piece and a large objective lens. Whereas a compound microscope has a large eye piece and small objective lens. Therefore can we use astronomical telescope as ...
0
votes
0answers
137 views

Universal curve of inelastic mean free path - why large at low energy?

I have seen the "universal" curve of inelastic mean free path (MFP) of electrons in many places (both experimental data, and just sketches of the curve) e.g. https://en.wikipedia.org/wiki/...
2
votes
2answers
49 views

How can I focus an optical device at infinity, inside a room with limited space?

I have a custom coupled camera/spectrograph imaging system that I need to focus at infinity for a microscope setup. I can't move the device outside, where I would be able to use some distant object to ...
0
votes
1answer
20 views

Holographic magnification and the requirement of single wavelength coherent reference wave

How is holographic magnification for different wavelength reference beams consistent with the requirement of illuminating the hologram with light with wavelength same as the reference beam? If we ...
0
votes
1answer
18 views

Near-Field Scanning Optical Microscope

Is it possible to study chemistry of a material (Like chemical composition) using Near-Field Scanning Optical Microscope? Is it possible to go below 10nm in NSOM/SNOM?
0
votes
1answer
293 views

Focal length vs working distance in an infinity corrected objective

Consider the following microscope objective (infinity corrected): Magnification $= 50 \times$ Glass Thickness $= 3.5$mm NA $= 0.50$ WD $= 13.89$mm Focal Length $= 4$mm I'm confused about the ...
0
votes
2answers
96 views

How to interpret and calculate scattering in a dark-field microscopy?

Dark-field microscopy setup from wikipedia, which says the images in dark-field microscopy are scattering lights from the sample. My questions: When the sample is illuminated, is it correct to ...
0
votes
1answer
52 views

How does the coating work when using an electron microscope?

When a non-conductive sample is viewed with an electron microscope, it can happen that some electrons from the beam can be trapped (absorbed) by the sample so it acquires a charge. This is called "...
3
votes
1answer
197 views

Derive resolving power of Heisenberg Microscope

Heisenberg's microscope used to demonstrate the Observer Effect/Uncertainty Principle begins by stating that the resolving power of any microscope is $$\Delta x=\frac{\lambda}{\sin \epsilon}$$ Being $\...
0
votes
2answers
178 views

why do we use a microscope to observe newton's ring? Can short focal distance telescope be used instead?

In newtons ring experiment we place the lens setup below a microscope which is then moved vertically until the fringes can be seen. In case of Determination of Cauchy's constants, we use a ...
-2
votes
1answer
167 views

What's the difference between the function of draw tube, coarse adjustment screws and fine adjustment screws? [closed]

I am having trouble with taking in the particular functions of draw tube, coarse and fine adjustment screws. The book that I read says: The Draw tube can be drawn up or down to focus the image. The ...
0
votes
1answer
83 views

Electron Microscopy: Are photons smaller than electrons?

So, I'm told that electron microscopy provides greater resolution than traditional photo/optical (i.e. visible light) microscopy, due to the (ahem) "fact" that "electrons are physically smaller than ...
0
votes
0answers
16 views

Relation between shorter exposure times and electron energy in electron microscopy?

When using electron microscopy for imaging biological samples, higher resolutions require shorter wavelengths, which require higher energies, which can easily destroy biological cells. This is ...
0
votes
1answer
35 views

Probe forming angle and resolution of a Scanning Electron Microscope

A SEM operates at $30keV$ and uses an objective lens with spherical aberration constant, $C_s=2mm$. Calculate the optimal probe forming angle for this system. Calculate the resolution of ...
1
vote
0answers
18 views

Generate oscillation (approx. f=50 HZ, Ampl=20 MikroMeter )

I want to adapt the height of a sample under a microscope. I have an x-y-positioner but no z-positioner. As a x-y-z-positioner is too expensive (about 16 k €) I want to build an own solution. First ...
0
votes
1answer
42 views

Characterization of graphene using optical microscopy

Number of layers of mechanically exfoliated graphite on silicon wafer can be estimated by observing through optical microscope. This is possible due to fact that huge colour contrast between different ...
0
votes
1answer
25 views

What, precisely and clearly, is the difference between scanning-tunneling microscopy and spectroscopy?

Is there a clearer description of the difference(s) between STM and STS than that on Wikipedia? I also found one other site which gave an equally contradictory, overlapping 'explanation'.
2
votes
0answers
81 views

Structured Illumination Microscopy Diffraction Grating Spacing How It Scales From SLM To Object Plane

I'm trying to understand better how to scale the pattern one generates on the Structured Light Modulator (SLM) into the object plane. I've been viewing various papers like the following one, but I ...
1
vote
1answer
26 views

STtimulated Emission Depletion (STED) microscopy theory

I am recently reading STED microscopy. In the Wiki, STED is described to be a process that the atoms of the flurophore in the periphery of the focal point is stimulated to emit light of wavelength ...
2
votes
0answers
50 views

How is the needle/tip of an STM actually made?

I was wondering how the needle/tip of an STM is made. Any links would be also great, I couldn't find any.
1
vote
0answers
52 views

magnification of objective lens of microscope

The magnification of objective lens of microscope is tube length divided by focal length of the objective lense. But Serway and Halliday give different definitions of tube length. One says the tube ...
1
vote
0answers
25 views

Will a condenser lens improve contrast under low magnification bright field imaging (10x)?

I have a 10x 0.28NA air lens that I am using to image inhomogeneities a semi-opaque gel cylinder - 500um in length and 100um in thickness, using white bright field light. Will a condenser lens have a ...
0
votes
1answer
39 views

Which interaction is responsible for secondary electron emission?

When imaging with SEM (Scanning Electron Microscopy) an incident electron ejects an electron from the surface of the sample, which is then detected in order to create an image. In that case I guess ...
0
votes
0answers
22 views

How can we predict the growth mode that a certain material will follow when we grow thin film?

we have 3 growth modes: Frank-van-der-Merwe, Stranski–Krastanov, Volmer-Weber surface science tag is still not available. so I use microscopy.
1
vote
0answers
65 views

What is the pixel size of Hitachi SU8230 SEM at 100k magnification?

I have a publication showing an SEM micrograph taken by Hitachi SU8230 model. Is there anyway to learn the pixel sizes?
0
votes
1answer
25 views

Magnifying glasses

In very simple terms, I understand that magnification occurs when light refracts through a biconvex lense producing a larger image on our retinas. But why is there no magnification if the object is ...
4
votes
1answer
128 views

How do electron microscopes not get obstructed by atoms in the air? [closed]

How do different electron microscopes avoid just scanning the atoms between the probe and the surface of the object that is actually being scanned?
0
votes
1answer
78 views

Magnification in Holographic Microscopy

The book "Introduction to Optics" by Frank, Leno, and Leno Pedrotti, Ed 3, makes the claim that It can be shown that if the reconstructing light of wavelength $\lambda_r$ is longer than the ...
0
votes
1answer
139 views

Thermonic Electron Gun

On the first figure, you can see schematic diagram of typical thermionic electron gun from Wiki.with filament voltage(1-2) electrons are emitted and with bias(1-3) - focused. and here is filament ...
2
votes
0answers
68 views

What is the bright spot in reflective digital holographic microscopy and how to remove it?

We are working on reflective DHM and the system looks like the setup in figure 1. We are facing a severe problem of reflection in the system. Apart from the reference beam and object beam, there is ...
2
votes
0answers
35 views

Brillouin Peak Localisation - Brillouin Imaging/Spectro-microscopy

I'm currently looking at ways of identifying the Stokes and anti-Stokes peaks in a Brillouin spectrum in the absence of Rayleigh peaks. The spectrum sort of looks like the shown below, but lacks the ...
1
vote
2answers
75 views

Why does and object need to be in focus when taking a picture? [closed]

As we know from junior high school physics, when an object is at twice of focal length f, it could form a same size real image. When an object is placed far from the lens, it would form a smaller ...
0
votes
0answers
230 views

How does a microscope objective lens work?

How does the magnification or image distance change when the object is placed much before the working distance in a semi-apochromatic or an apochromatic lens? I am considering just the objective and ...
0
votes
1answer
155 views

Rayleigh Criterion for the Lateral Resolution of the AFM

I was reviewing my labnotes and got stuck on one question: The finite dimension and size of the cantilever tip has an effect on the appearance and size of the images. Tip broadening will occur when ...
2
votes
1answer
81 views

How does the decay of an atom look like?

There is this famous image recorded by IBM using the Scanning tunneling microscope. Those dots are individual Xenon atoms. I was thinking if it was possible to record the sequence of images of any ...
0
votes
0answers
44 views

Why could we interact with the physical world quicker using nanotechnology?

I'm reading a book on artificial intelligence. Given a scenario where a superintelligent mind exists with 10.000 times the speed of a human brain, technological progress would be hindered by the ...
2
votes
1answer
106 views

Any differences between critical dimension/size and resolution for an optical objective?

I were studying something about the diffraction limit for a microscope when i found this definition: For a focusing objective or for lithography applications, the smallest resolvable distance or ...
0
votes
2answers
51 views

monochromatic aberrations shown as different color on screen?

Below a picture of an 8mm ball bearing taken with a blue LED back light (447.5nm). I assume what we see here is a diffraction pattern, and the blue/yellow banding is due to chromatic aberration. I ...
0
votes
1answer
139 views

True white collimated light / nobody uses this?

Based on a previous question is this the difraction limit of our microscope? , I got the idea to use a true white parallel back light to illuminate an object for measurements (target accuracy is 1 ...
1
vote
1answer
329 views

Why would an infinity corrected objective lens produce an image without a tube lens?

I'm having a hard time wrapping my head around this situation I've come across. I'm essentially recreating a homemade microscope that my understanding would suggest shouldn't work. This setup uses a ...
3
votes
1answer
178 views

What is the likely source of these interference/noise patterns in my DIY laser micro-projector?

A little background: I've chopped up a small LCoS "Pico Projector" and turned it into a micro-projector. E.g. I removed all the projection lenses and replaced them with basically a microscope ...
1
vote
2answers
350 views

What type of LED to use with a 100 micrometer pinhole for in-line digital holography?

I want to set up an in-line digital holographic microscope that is similar to the one describe in the article, A Study of Digital In-Line Holographic Microscopy for Malaria Detection, which is they ...
1
vote
1answer
2k views

Ray diagram of focussing on a compound microscope

When we use a usual biology class compound microscope, we need to focus the stage (place the object) to the "correct" position. It is just in that sweet spot that the image is sharp and clear. So we ...
0
votes
0answers
34 views

Various components in Transmission Electron Microscope (Reference)

I am new to Transmission Electron Microscope and tried googling to find out some references which has lucid description about various components used in TEM,like ETH Zurich sites , still any help in ...
5
votes
1answer
1k views

Why don't we have proton/neutron microscopes?

According to wave-particle duality it should be possible for protons/neutrons to have wavelengths shorter than those of electrons having similar velocity. So why don't we use proton/neutron ...