Questions tagged [microscopy]
The microscopy tag has no usage guidance.
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Why would an object disappear when switching from monocular to binocular vision?
I have just used a compound binocular microscope which has an ‘eyepiece graticule’ (ruler in arbitrary units) in the right eyepiece lens.
If you close your left eye (or occlude the left lens), the ...
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Lowest numerical aperture SIM microscopy
What is the lowest numerical aperture objective that can support SIM microscopy? Also, how does the NA affect the spatial frequency or contrast of the resultant standing wave?
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Is it possible to make real DIY microscope with several lens?
I have alot of small double convex 7 mm lenses and i have very small lens 4mm
And i have plano concave lenses all of them is small
I tried to combining them to get a magnified image and it worked when ...
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Trouble simulating/ray tracing a simple microscope
I'm trying to run a (seemingly) simple simulation of a basic microscope using the free software WinLens 3D Basic, but the ray tracing looks wrong:
The microscope consists of a small plano-convex lens ...
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Why do fluorescence microscopes use long-pass dichroics
Superresolution fluorescence microscopy is very sensitive to chromatic aberration. Dichroic mirrors, while being biplanar, are not normal to the optical axis and so will cause some dispersion in one ...
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Anomalous Spectral Function and STM
I have a question in regard to the anomalous spectral function and how it pertains to STM experiments. The use of the spectral representation of Green functions is assumed known, so I do not go into ...
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Question in Linear response theory
I am currently trying to do a calculation using linear response, but I am confused on one thing I never thought of. To start here is the relevant equation (I assumed the operators are self evident to ...
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Near Field Optical Microscope
As a handyman, once I took pictures of a corner of a parfaim bottle by my camera (Near Field Optical Microscope), I'd like to know the interpretation of the following image:
less powerful zoom:
https:/...
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Why can’t we see atoms in an optical microscope?
I know, the answer to this question may seem obvious: The resolution/magnification of an optical microscope is limited by the minimum wavelength one uses. This is due to the diffraction limit.
However,...
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Equivalent absorbed radiation dose exposed to Electron Microscopy Beam
I'm intentionally exposed an Integrated Circuit (IC) to electron beam under a SEM Microscope. I have different accelerating voltages and different current beam parameters to be adjusted.
For example, ...
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Why the broadening of coherence peak in superconducting spectrum is described by an imaginary term?
From Phys. Rev. Lett. 41 1509, the density of states of superconductor is
where iΓ is the broadening part.
Can anyone tell me why the broadening can be decribed by an imaginary part, what is its ...
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Aperture/image circle in a DIY digital microscope?
I have just taken apart a broken USB microscope, and recognized that there is actually no magic going on inside.
So I thought how about building one of my own. The idea is to buy a regular (possibly ...
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(Optical microscopy) Resolution improvement by using oil immersion
'Oil-immersion' technique is widely used to increase the numerical aperture, and hence the resolution in optical microscopy.
Some microscope manufactures like Nikon or ZEISS explain the principle of ...
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Can you use a digital camera CCD to make an image without a lens?
What would you be able to see if you took off the lens of a digital camera and placed a drop of pond water containing microbes/amoebas directly on the sensor? Would you be able to see an image? The ...
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Sharpness of images on specular vs diffuse surfaces
I'm observing a confusing phenomenon where I'm projecting an image episcopically through an objective into a substrate while observing it through a camera (think structured illumination for a ...
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STED Microscopy: Differentiate between emission from stimulated emission and fluorescence
From what I understand about stimulated emission in STED microscopy, a red shifted Laser (apperently with a wavelength around the emission wavelength of the fluorophor) is used to induce stimulated ...
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Laser confocal microscope filter
I wanted to mount a narrow bandpass filter which allows to transmit 405 nm wave, i. e. the laser wavelength. I wanted to cut off other light, cause my detector is also sensitive to other wavelengths.
...
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How do you interpret this SEM image? [duplicate]
[]can you help us in interpreting this image?1
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Does the substage condenser numerical aperture cap total resolution of an imaging system?
Amongst formulas to estimate the theoretical resolution of a transmitted light microscope, I often see:
$$
\delta = \frac{1.22\lambda}{NA_{obj} + NA_{cond}}
$$
Where $NA_{cond}$ is the numerical ...
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How can I magnify an infinity corrected image onto a camera surface?
I know that the typical setup with a camera will place the CMOS sensor at I(3) in the image below, and that this magnification is dictated by the tube lens focal ...
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How do I read a scanning electron microscopy (SEM) image and an element of the photo
I'm trying to figure out the information I can take of this image. What I did found out is the following information :
The increase is 600 times.
The voltage is 30.1 kilowatts.
The relief corresponds ...
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Operation of a microscope and image reconstruction
I have a question about the operation of the optical microscope. With reference to the image:
from what I know, the image of the object is formed the first time where the red arrow is, why then is ...
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In a Mirau Interferometer what is the distance between the mirror and the beamsplitter?
A Mirau interferometer (shown left in the figure, next to the Michelson configuration to the right) is an important instrument for non-contact surface profilometry.
Briefly, taken from Wikipedia, &...
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What would happen is I bring an n-type semiconductor in contact with a positive charge?
I am currently doing an experiment where I have a pyramidal n-type semiconductor attached to an insulator. This apparatus is brought into contact with a charged surface having positive and negative ...
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Is it possible to achieve Atomic resolution with the following parameters of a Scanning Probe Microscope? [closed]
I understand that the formula for Resolution is the Dimension of the image/Dimension of the object, however I am still unable to solve this. Thanks for any help in advance
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Formula for total magnification of a compound microscope
In the book 'Materials Characterization: Introduction to Microscopic and Spectroscopic Methods, Second Edition pdf' its says:
The total magnification of a compound microscope as shown in Figure 1.1 ...
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How can can evanescent field cause fluorescence if there is no energy transport?
I am currently looking at the topic "Total Internal Reflection Microscopy".
This creates an evanescent field travelling to my sample. This evanescent field no activates the Fluorophores. But ...
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How does coherence of a light affect how we perceive a sample in a microscope?
First of all, does coherence of a light, which is used for illumination in a microscope, affect the sample, or the way we see/perceive it? I want to know what optical elements do I need to use if I ...
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Why Airy disk (Fraunhofer diffraction) is first order of the Bessel function of the first kind?
I have been studying Fourier ptychography.
I am confused about why Airy disk is J1 of the Bessel function. I think that Fraunhofer diffraction looks more like J0 of the Bessel function.
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How to set mask size when apply inverse-FFT to the power spectrum of HAADF-STEM image?
Hello everyone, I want to characterize dislocation in my FIB sample using Titan electron transmission microscopy and I want to use FFT filter to process my HAADF-STEM image with DigitalMicrograph (...
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Cryogenic microscope objective
Are the Attocube LT-APO cryogenic objectives with a numerical aperture of 0.82 actually suitable for work with single quantum emitters at liquid helium temperatures? If not, are there any other ...
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Could a reflective beam expander deteriorate the lateral symmetry of a beam collimated from a single-mode optical fiber?
Background: I am currently concerned with getting a confocal time-correlated single-photon counting (TCSPC) setup ready for autocorrelation spectroscopy (fundamentally: fluorescence autocorrelation (...
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How exactly can you "grab" a single atom with a scanning tunneling microscope?
I have read many questions on this site and elsewhere about STM's but none of them specifically talk about how they actually "grab" and move the single atoms.
The best I found, is saying ...
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Coherent and incoherent electron diffraction
I am learning about electron diffraction in a transmission electron microscope and was wondering about following topic:
A single crystalline material will exhibit a diffraction pattern with discrete ...
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Why is the resolution of a tunneling microscope not limited by the wavelength of the electrons?
Why is the resolution of a tunneling microscope not limited by the wavelength of the electrons? Is it impossible for the electrons that are tunneling across the gap to appear somewhere in the gap, ...
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Minimum Tube length distance between objective lens to tube lens
The maximum tube length between objective lens → tube lens (reference here)
MaxTubeLength = (TubeLensPupilDia-ObjectiveExitPupilDia) x (TubeLens_FL/SensorDiagonal)
You can use the calculator here. ...
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Objective lens and Eyepiece of a compound microscope
Why is the objective lens always powerful than Eyepiece of a compound microscope?
Will it not work if Eyepiece is more powerful?
In a book it is given that Eyepiece has larger focal length.
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Is energy not conserved in a microscope?
The Point Spread Function (PSF) of an imaging system is its impulse response and defines how a single point on the sample plane is spreaded on the image plane. The simplest model is the one calculated ...
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Magnetic detector for electron beam
I want to create a mathematical model for a magnetic electron beam detector.
It is composed by an air gap inductor with inductance $L$ and distance $d$.
An electron beam with Energy $E_b$ and number ...
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Why should dichroic mirrors be placed observing a certain orientation, and what happens otherwise?
I am getting very confused about the reason why dichroic mirrors should be placed observing a certain orientation. I read everywhere that you should place the mirror with the coated side facing the ...
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Is there a phase contrast method for SEM?
I was searching in the literature, and I did not find any phase contrast method for SEM microscopy. I would like to to if there is any, and if it not why?
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How microscopy could be considered as convolution?
I just read here that "The process of how a lens, or objective, forms an image of any object is called convolution."
When we are talking about convolution, I expect something like this:
$$(f*...
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Why does magnification work?
I have read the question How do telescopes work as magnifiers? but it doesn't quite address, as far as I can tell, my question.
I'm trying to wrap my head around why magnification works rather than ...
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How do electromagnetic lenses magnify the image?
In optical microscope both objective and eyepiece are used to magnify the sample image. Magnification is determined by laws of geometrical optics (intersection of optical beams from the same point of ...
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Microscope and dual nature of light
Does a light microscope also prove the particle nature of light?
As in electron microscope there is either transmission or absorbance of electrons to create an image, hence the question above!
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Why a mode-locked reduce the integration time in microscopy?
Why a mode-locked (laser) reduce the "integration time" in two-photon fluorescence microscopy?
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What is integration time in microscopy?
What is the "integration time" in Two-photon excitation microscopy?
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Whats "integration time" in two photon microscopy?
What is the meaning "integration time" in Two-photon excitation microscopy?
Why a mode-locked signal (laser) can reduce the "integration time" in Two-photon excitation microscopy?
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Measuring temperature at submicron resolution
Do we have any microscopic technique/protocol to measure temperature at sub-micron resolution?
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Scanning electron microscopy (SEM) images without Conductive Samples
The traditional understanding for obtaining scanning electron microscopy (SEM) images is that one first needs to coat the specimen in gold (or similar).
This is supposedly necessary because biological ...
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