Stack Exchange Network

Stack Exchange network consists of 174 Q&A communities including Stack Overflow, the largest, most trusted online community for developers to learn, share their knowledge, and build their careers.

Visit Stack Exchange

Questions tagged [microscopy]

The tag has no usage guidance.

0
votes
0answers
29 views

Microscopic bible , how was it made? [closed]

I once saw a video that during communism or some other era, people used to somehow print whole bibles on a microscopic slide. How could this possibly have been done? Unfortunately i can't find ...
0
votes
1answer
54 views

Can telescope have dual purpose? [duplicate]

Astronomical telescope has a small eye piece and a large objective lens. Whereas a compound microscope has a large eye piece and small objective lens. Therefore can we use astronomical telescope as ...
0
votes
0answers
76 views

Universal curve of inelastic mean free path - why large at low energy?

I have seen the "universal" curve of inelastic mean free path (MFP) of electrons in many places (both experimental data, and just sketches of the curve) e.g. https://en.wikipedia.org/wiki/...
-1
votes
0answers
7 views

In a compound microscope what happens if the focal length of the objective lens is increased?

My textbook says that the magnifying power will decrease but doesn't specify exactly how?
2
votes
2answers
46 views

How can I focus an optical device at infinity, inside a room with limited space?

I have a custom coupled camera/spectrograph imaging system that I need to focus at infinity for a microscope setup. I can't move the device outside, where I would be able to use some distant object to ...
0
votes
1answer
19 views

Holographic magnification and the requirement of single wavelength coherent reference wave

How is holographic magnification for different wavelength reference beams consistent with the requirement of illuminating the hologram with light with wavelength same as the reference beam? If we ...
0
votes
1answer
14 views

Near-Field Scanning Optical Microscope

Is it possible to study chemistry of a material (Like chemical composition) using Near-Field Scanning Optical Microscope? Is it possible to go below 10nm in NSOM/SNOM?
0
votes
0answers
40 views

Shifting focal point with lens

I have an reflective microscope objective in my microscope (namely this one: https://www.newport.com/p/50105-02). Without moving the objective I need to slightly shift the focal point further away ...
-1
votes
0answers
12 views

How to check for liquid surfaces with Atomic Force Microscopy

So, I'm helping a teacher with a project, and we are using AFM to check a certain polymer's mechanical characteristics, but as an "extra" question I was supposed to try and measure the presence of ...
0
votes
1answer
84 views

Focal lenght vs working distance in an infinity corrected objective

Consider the following microscope objective (infinity corrected): Magnification $= 50 \times$ Glass Thickness $= 3.5$mm NA $= 0.50$ WD $= 13.89$mm Focal Length $= 4$mm I'm confused about the ...
0
votes
2answers
63 views

How to interpret and calculate scattering in a dark-field microscopy?

Dark-field microscopy setup from wikipedia, which says the images in dark-field microscopy are scattering lights from the sample. My questions: When the sample is illuminated, is it correct to ...
0
votes
1answer
51 views

How does the coating work when using an electron microscope?

When a non-conductive sample is viewed with an electron microscope, it can happen that some electrons from the beam can be trapped (absorbed) by the sample so it acquires a charge. This is called "...
0
votes
0answers
28 views

Hyper-Resolution Optical Microscopy

Can anyone tell what is the fundamental limit for using image processing to do a de-convolution of the airy disk in optical microscopy? In order to simplify this operation why not determine the ...
0
votes
0answers
17 views

Field of view of an optical microscope - Definition and example (150 x 150 micrometer)

What is the definition of field of view of an optical microscope? Can you show me also an example please? I read that a microscope has a field of view of 150 x 150 micrometer; what does it mean? ...
3
votes
1answer
116 views

Derive resolving power of Heisenberg Microscope

Heisenberg's microscope used to demonstrate the Observer Effect/Uncertainty Principle begins by stating that the resolving power of any microscope is $$\Delta x=\frac{\lambda}{\sin \epsilon}$$ Being $\...
0
votes
2answers
118 views

why do we use a microscope to observe newton's ring? Can short focal distance telescope be used instead?

In newtons ring experiment we place the lens setup below a microscope which is then moved vertically until the fringes can be seen. In case of Determination of Cauchy's constants, we use a ...
-2
votes
1answer
76 views

What's the difference between the function of draw tube, coarse adjustment screws and fine adjustment screws? [closed]

I am having trouble with taking in the particular functions of draw tube, coarse and fine adjustment screws. The book that I read says: The Draw tube can be drawn up or down to focus the image. The ...
0
votes
1answer
78 views

Electron Microscopy: Are photons smaller than electrons?

So, I'm told that electron microscopy provides greater resolution than traditional photo/optical (i.e. visible light) microscopy, due to the (ahem) "fact" that "electrons are physically smaller than ...
0
votes
0answers
16 views

Relation between shorter exposure times and electron energy in electron microscopy?

When using electron microscopy for imaging biological samples, higher resolutions require shorter wavelengths, which require higher energies, which can easily destroy biological cells. This is ...
0
votes
1answer
28 views

Probe forming angle and resolution of a Scanning Electron Microscope

A SEM operates at $30keV$ and uses an objective lens with spherical aberration constant, $C_s=2mm$. Calculate the optimal probe forming angle for this system. Calculate the resolution of ...
1
vote
0answers
17 views

Generate oscillation (approx. f=50 HZ, Ampl=20 MikroMeter )

I want to adapt the height of a sample under a microscope. I have an x-y-positioner but no z-positioner. As a x-y-z-positioner is too expensive (about 16 k €) I want to build an own solution. First ...
0
votes
1answer
36 views

Characterization of graphene using optical microscopy

Number of layers of mechanically exfoliated graphite on silicon wafer can be estimated by observing through optical microscope. This is possible due to fact that huge colour contrast between different ...
0
votes
1answer
24 views

What, precisely and clearly, is the difference between scanning-tunneling microscopy and spectroscopy?

Is there a clearer description of the difference(s) between STM and STS than that on Wikipedia? I also found one other site which gave an equally contradictory, overlapping 'explanation'.
2
votes
0answers
72 views

Structured Illumination Microscopy Diffraction Grating Spacing How It Scales From SLM To Object Plane

I'm trying to understand better how to scale the pattern one generates on the Structured Light Modulator (SLM) into the object plane. I've been viewing various papers like the following one, but I ...
1
vote
1answer
24 views

STtimulated Emission Depletion (STED) microscopy theory

I am recently reading STED microscopy. In the Wiki, STED is described to be a process that the atoms of the flurophore in the periphery of the focal point is stimulated to emit light of wavelength ...
2
votes
0answers
47 views

How is the needle/tip of an STM actually made?

I was wondering how the needle/tip of an STM is made. Any links would be also great, I couldn't find any.
1
vote
0answers
47 views

magnification of objective lens of microscope

The magnification of objective lens of microscope is tube length divided by focal length of the objective lense. But Serway and Halliday give different definitions of tube length. One says the tube ...
1
vote
0answers
25 views

Will a condenser lens improve contrast under low magnification bright field imaging (10x)?

I have a 10x 0.28NA air lens that I am using to image inhomogeneities a semi-opaque gel cylinder - 500um in length and 100um in thickness, using white bright field light. Will a condenser lens have a ...
0
votes
1answer
37 views

Which interaction is responsible for secondary electron emission?

When imaging with SEM (Scanning Electron Microscopy) an incident electron ejects an electron from the surface of the sample, which is then detected in order to create an image. In that case I guess ...
0
votes
0answers
22 views

How can we predict the growth mode that a certain material will follow when we grow thin film?

we have 3 growth modes: Frank-van-der-Merwe, Stranski–Krastanov, Volmer-Weber surface science tag is still not available. so I use microscopy.
0
votes
0answers
34 views

Dark-field Spectroscopy Setup Help

Having issues obtaining meaningful dark-field spectra. Using an inverted microscope, with a darkfield condenser and a 12V halogen lamp. I focus the cone of light on the specimen, and use an 100x 0....
1
vote
0answers
62 views

What is the pixel size of Hitachi SU8230 SEM at 100k magnification?

I have a publication showing an SEM micrograph taken by Hitachi SU8230 model. Is there anyway to learn the pixel sizes?
0
votes
0answers
40 views

Laser Damage Threshold (LDT) for a MEMS mirror

A MEMS mirror I want to use is rated to withstand 2W of continuous wave laser power (visible light). The mirror itself is made out of single-crystal silicon structure that is 50 um (micrometer) thick ...
0
votes
1answer
24 views

Magnifying glasses

In very simple terms, I understand that magnification occurs when light refracts through a biconvex lense producing a larger image on our retinas. But why is there no magnification if the object is ...
4
votes
1answer
126 views

How do electron microscopes not get obstructed by atoms in the air? [closed]

How do different electron microscopes avoid just scanning the atoms between the probe and the surface of the object that is actually being scanned?
0
votes
1answer
76 views

Magnification in Holographic Microscopy

The book "Introduction to Optics" by Frank, Leno, and Leno Pedrotti, Ed 3, makes the claim that It can be shown that if the reconstructing light of wavelength $\lambda_r$ is longer than the ...
0
votes
0answers
28 views

Falaco solitons at the cellular level?

Falaco solitons are unique in their longevity,reportedly up to 15 minutes on the surface of a body of still water. For details and the math see {{ http://www22.pair.com/csdc/pdf/pdf/falsol.pdf}} In ...
0
votes
1answer
121 views

Thermonic Electron Gun

On the first figure, you can see schematic diagram of typical thermionic electron gun from Wiki.with filament voltage(1-2) electrons are emitted and with bias(1-3) - focused. and here is filament ...
2
votes
0answers
67 views

What is the bright spot in reflective digital holographic microscopy and how to remove it?

We are working on reflective DHM and the system looks like the setup in figure 1. We are facing a severe problem of reflection in the system. Apart from the reference beam and object beam, there is ...
2
votes
0answers
34 views

Brillouin Peak Localisation - Brillouin Imaging/Spectro-microscopy

I'm currently looking at ways of identifying the Stokes and anti-Stokes peaks in a Brillouin spectrum in the absence of Rayleigh peaks. The spectrum sort of looks like the shown below, but lacks the ...
1
vote
2answers
74 views

Why does and object need to be in focus when taking a picture? [closed]

As we know from junior high school physics, when an object is at twice of focal length f, it could form a same size real image. When an object is placed far from the lens, it would form a smaller ...
0
votes
0answers
205 views

How does a microscope objective lens work?

How does the magnification or image distance change when the object is placed much before the working distance in a semi-apochromatic or an apochromatic lens? I am considering just the objective and ...
0
votes
1answer
143 views

Rayleigh Criterion for the Lateral Resolution of the AFM

I was reviewing my labnotes and got stuck on one question: The finite dimension and size of the cantilever tip has an effect on the appearance and size of the images. Tip broadening will occur when ...
2
votes
1answer
77 views

How does the decay of an atom look like?

There is this famous image recorded by IBM using the Scanning tunneling microscope. Those dots are individual Xenon atoms. I was thinking if it was possible to record the sequence of images of any ...
0
votes
0answers
41 views

Why could we interact with the physical world quicker using nanotechnology?

I'm reading a book on artificial intelligence. Given a scenario where a superintelligent mind exists with 10.000 times the speed of a human brain, technological progress would be hindered by the ...
2
votes
1answer
96 views

Any differences between critical dimension/size and resolution for an optical objective?

I were studying something about the diffraction limit for a microscope when i found this definition: For a focusing objective or for lithography applications, the smallest resolvable distance or ...
0
votes
0answers
131 views

Orthogonalization of a hexagonal unit cell

I am using the Multislice technique to produce simulated TEM images. For Graphene, the unit cell is no longer rectangular but hexagonal. I have data for the projected potential in each rhombic slice ...
0
votes
2answers
48 views

monochromatic aberrations shown as different color on screen?

Below a picture of an 8mm ball bearing taken with a blue LED back light (447.5nm). I assume what we see here is a diffraction pattern, and the blue/yellow banding is due to chromatic aberration. I ...
0
votes
1answer
114 views

True white collimated light / nobody uses this?

Based on a previous question is this the difraction limit of our microscope? , I got the idea to use a true white parallel back light to illuminate an object for measurements (target accuracy is 1 ...
1
vote
1answer
268 views

Why would an infinity corrected objective lens produce an image without a tube lens?

I'm having a hard time wrapping my head around this situation I've come across. I'm essentially recreating a homemade microscope that my understanding would suggest shouldn't work. This setup uses a ...