Questions tagged [microscopy]

The tag has no usage guidance.

Filter by
Sorted by
Tagged with
0 votes
0 answers
7 views

STM microscope kinematic movement [closed]

I found the project for STM microscope, where kinematic movement is regulated with three fine adjustment screws: STM scan head. I own a double frame with two fine adjustment screws on diagonal and the ...
0 votes
0 answers
23 views

In a Mirau Interferometer what is the distance between the mirror and the beamsplitter?

A Mirau interferometer (shown left in the figure, next to the Michelson configuration to the right) is an important instrument for non-contact surface profilometry. Briefly, taken from Wikipedia, &...
0 votes
0 answers
18 views

What would happen is I bring an n-type semiconductor in contact with a positive charge?

I am currently doing an experiment where I have a pyramidal n-type semiconductor attached to an insulator. This apparatus is brought into contact with a charged surface having positive and negative ...
  • 1
1 vote
0 answers
12 views

Is it possible to achieve Atomic resolution with the following parameters of a Scanning Probe Microscope? [closed]

I understand that the formula for Resolution is the Dimension of the image/Dimension of the object, however I am still unable to solve this. Thanks for any help in advance
  • 11
0 votes
1 answer
23 views

Formula for total magnification of a compound microscope

In the book 'Materials Characterization: Introduction to Microscopic and Spectroscopic Methods, Second Edition pdf' its says: The total magnification of a compound microscope as shown in Figure 1.1 ...
1 vote
0 answers
29 views

How can can evanescent field cause fluorescence if there is no energy transport?

I am currently looking at the topic "Total Internal Reflection Microscopy". This creates an evanescent field travelling to my sample. This evanescent field no activates the Fluorophores. But ...
  • 31
0 votes
0 answers
22 views

How does coherence of a light affect how we perceive a sample in a microscope?

First of all, does coherence of a light, which is used for illumination in a microscope, affect the sample, or the way we see/perceive it? I want to know what optical elements do I need to use if I ...
  • 1
0 votes
1 answer
77 views

Why Airy disk (Fraunhofer diffraction) is first order of the Bessel function of the first kind?

I have been studying Fourier ptychography. I am confused about why Airy disk is J1 of the Bessel function. I think that Fraunhofer diffraction looks more like J0 of the Bessel function.
1 vote
1 answer
51 views

How to set mask size when apply inverse-FFT to the power spectrum of HAADF-STEM image?

Hello everyone, I want to characterize dislocation in my FIB sample using Titan electron transmission microscopy and I want to use FFT filter to process my HAADF-STEM image with DigitalMicrograph (...
  • 11
0 votes
0 answers
7 views

What type of scattering happens in dark field optical microscope?

I know that in dark field optical microscope, the light scatters off the specimen under an angle and falls on the detector. I am curious if the scattering that is taking place there is considered to ...
  • 93
0 votes
1 answer
45 views

Cryogenic microscope objective

Are the Attocube LT-APO cryogenic objectives with a numerical aperture of 0.82 actually suitable for work with single quantum emitters at liquid helium temperatures? If not, are there any other ...
0 votes
0 answers
10 views

Contact AFM/SPM perceived depth help

If a multi-walled nanotube (diameter = 5 mm) is lying on an atomically flat substrate and being imaged in contact mode, by a tip that's terminated at the apex by a spherical section of 2.5 mm radius ...
0 votes
0 answers
27 views

Could a reflective beam expander deteriorate the lateral symmetry of a beam collimated from a single-mode optical fiber?

Background: I am currently concerned with getting a confocal time-correlated single-photon counting (TCSPC) setup ready for autocorrelation spectroscopy (fundamentally: fluorescence autocorrelation (...
0 votes
1 answer
49 views

How exactly can you "grab" a single atom with a scanning tunneling microscope?

I have read many questions on this site and elsewhere about STM's but none of them specifically talk about how they actually "grab" and move the single atoms. The best I found, is saying ...
0 votes
0 answers
29 views

Coherent and incoherent electron diffraction

I am learning about electron diffraction in a transmission electron microscope and was wondering about following topic: A single crystalline material will exhibit a diffraction pattern with discrete ...
0 votes
1 answer
50 views

Why is the resolution of a tunneling microscope not limited by the wavelength of the electrons?

Why is the resolution of a tunneling microscope not limited by the wavelength of the electrons? Is it impossible for the electrons that are tunneling across the gap to appear somewhere in the gap, ...
  • 2,218
0 votes
0 answers
130 views

Minimum Tube length distance between objective lens to tube lens

The maximum tube length between objective lens → tube lens (reference here) MaxTubeLength = (TubeLensPupilDia-ObjectiveExitPupilDia) x (TubeLens_FL/SensorDiagonal) You can use the calculator here. ...
1 vote
0 answers
28 views

Objective lens and Eyepiece of a compound microscope

Why is the objective lens always powerful than Eyepiece of a compound microscope? Will it not work if Eyepiece is more powerful? In a book it is given that Eyepiece has larger focal length.
  • 13
1 vote
0 answers
77 views

Is energy not conserved in a microscope?

The Point Spread Function (PSF) of an imaging system is its impulse response and defines how a single point on the sample plane is spreaded on the image plane. The simplest model is the one calculated ...
1 vote
1 answer
67 views

Magnetic detector for electron beam

I want to create a mathematical model for a magnetic electron beam detector. It is composed by an air gap inductor with inductance $L$ and distance $d$. An electron beam with Energy $E_b$ and number ...
0 votes
0 answers
12 views

What's the voltage accuracy of tip electrode of modern SEMs (Scanning Electrode Microscope)?

I want to know with what accuracy the potential(or the voltage) of a tip electrode (of a SEM) can be adjusted?
  • 101
2 votes
1 answer
49 views

Why should dichroic mirrors be placed observing a certain orientation, and what happens otherwise?

I am getting very confused about the reason why dichroic mirrors should be placed observing a certain orientation. I read everywhere that you should place the mirror with the coated side facing the ...
  • 21
0 votes
0 answers
17 views

Is there a phase contrast method for SEM?

I was searching in the literature, and I did not find any phase contrast method for SEM microscopy. I would like to to if there is any, and if it not why?
  • 21
2 votes
0 answers
48 views

How microscopy could be considered as convolution?

I just read here that "The process of how a lens, or objective, forms an image of any object is called convolution." When we are talking about convolution, I expect something like this: $$(f*...
  • 21
3 votes
5 answers
143 views

Why does magnification work?

I have read the question How do telescopes work as magnifiers? but it doesn't quite address, as far as I can tell, my question. I'm trying to wrap my head around why magnification works rather than ...
1 vote
0 answers
39 views

How do electromagnetic lenses magnify the image?

In optical microscope both objective and eyepiece are used to magnify the sample image. Magnification is determined by laws of geometrical optics (intersection of optical beams from the same point of ...
0 votes
0 answers
11 views

Does the inelstic mean free path change with the doping concentration?

Does the bulk plasmon cross section depend on the doping concentration? I measured EELS for three different doping concentrations of silicon and the inelstic mean free path seems to increase ...
  • 1
3 votes
3 answers
108 views

Microscope and dual nature of light

Does a light microscope also prove the particle nature of light? As in electron microscope there is either transmission or absorbance of electrons to create an image, hence the question above!
1 vote
1 answer
40 views

Why a mode-locked reduce the integration time in microscopy?

Why a mode-locked (laser) reduce the "integration time" in two-photon fluorescence microscopy?
  • 43
2 votes
1 answer
81 views

What is integration time in microscopy?

What is the "integration time" in Two-photon excitation microscopy?
  • 43
1 vote
0 answers
20 views

Whats "integration time" in two photon microscopy?

What is the meaning "integration time" in Two-photon excitation microscopy? Why a mode-locked signal (laser) can reduce the "integration time" in Two-photon excitation microscopy?
  • 43
-2 votes
2 answers
45 views

Measuring temperature at submicron resolution

Do we have any microscopic technique/protocol to measure temperature at sub-micron resolution?
0 votes
0 answers
24 views

Scanning electron microscopy (SEM) images without Conductive Samples

The traditional understanding for obtaining scanning electron microscopy (SEM) images is that one first needs to coat the specimen in gold (or similar). This is supposedly necessary because biological ...
  • 812
0 votes
1 answer
50 views

Magnification of a system of lenses

I have a lens system consisting of 7 elements. However, I cannot take the system apart to measure the distance between them or their focal lengths. I do, however, know the effective focal length of ...
  • 11
0 votes
0 answers
25 views

Practical to measure topography of a large area (mm-scale) with scanning probe microscopy (SPM)?

I'm currently very new to nanotechnology. I do understand that it is possible to achieve an excellent topography image of a sample by SPM. However, is it possible to do SPM over a large area (for ...
0 votes
0 answers
26 views

Does it makes sense to interpret the Point Spread Function as a volumetric density of energy?

The Point Spread Function (PSF) of an imaging system is its impulse response. Therefore $$ I(x,y,z) = \left[O \star PSF\right](x,y,z) $$ where $I$ is the image, $O$ is the object, and $\star$ is the ...
2 votes
1 answer
114 views

Why do we observe ripples in Quantum corrals and Scanning Tunneling Microscope (STM), if the wavefunction isn't physically realizable?

I recently landed upon an image of Quantum corral and observed ripples around the atoms. Such pattern of ripples were also observed in a movie by IBM - A Boy and his Atom. Upon some research, I found ...
  • 1,228
1 vote
0 answers
15 views

Removing collected charges in a IC CMOS circuit exposed under a 30keV electron beam source

I would like to know if there is any mechanism (for example UV light exposition) to remove charges collected in an IC CMOS circuit that was exposed under a 30keV electron beam source. Presumably, ...
1 vote
0 answers
79 views

White light interferometer, how much precise must be?

I am trying to build a small interferometer, but after many attempt, I still can't see any fringes or interference effect. How much precise need to be the surfaces and the angles to see interference ...
  • 1,316
0 votes
1 answer
105 views

Magnification of a compound microscope

Whilst finding the magnification of a compound microscope, we multiply the linear magnification of the objective with the angular magnification of the eyepiece. This gives us the angular magnification ...
  • 5
1 vote
0 answers
28 views

Glow around objects in AFM image (amplitude modulation mode)

I have made an AFM scan (Amplitude modulation mode) of a sample consisting of polyethylene in a matrix of polystyrene. I have tried to figure out why there appears a 'glow' around the domes of ...
  • 241
0 votes
1 answer
48 views

What is the wavelength of a signal with a continuously increasing frequency?

What is the wavelength of a signal continuously decreasing in power (and seemingly decreasing in wavelength)? This question comes as a consequence of trying to find the correct value for the ...
1 vote
1 answer
76 views

Regarding measurements on traveling microscope

In a experiment with traveling microscope to determine thickness of a glass slab , what is the physical significance of the readings that we get from the vertical scale ?, Means What are two points ...
2 votes
1 answer
45 views

If ordinary electron microscopes have wavelengths 5,000 times or more shorter than visible light, why can't they see atoms?

Electron microscopes can easily 'see' down to .1 nanometers (1 ångstrom) or less, correct? And a single hydrogen atom, in its ground state, is about .106 nanometers (Bohr diameter) wide, right? So ...
  • 3,725
0 votes
2 answers
34 views

How can an electron's de Broglie wavelength be LONGER than .0024264 nanometers?

If the rest mass of an electron is about 510,998.5 ev, or .5109985 Mev, doesn't that mean that even the slowest-moving electron has a wavelength of no more than .0024264 nanons? That is the wavelength ...
  • 3,725
0 votes
1 answer
18 views

Is tunnel microscope destruct a bit the material, by extracting electrons from the probed material?

The tunnel microscope probes the electrons of a material by quantum tunneling effect. As a consequence of the migration of electrons, is the tunnel microscope "destroying" a bit the material,...
2 votes
1 answer
193 views

Larger aperture for objective lens in a compound microscope

A telescope has a large aperture to collect more light and hence improve the visibility (brightness) of the image. It also helps in improving the resolution as $\theta_{\text{min}}=\frac{1.22\lambda}{...
1 vote
1 answer
60 views

What's the difference between "optical amplification" and "magnifying"?

Optical amplification used widely in astronomical observatories and "magnifying" used in microscopes. What's the difference between "optical amplification" and "magnifying&...
1 vote
1 answer
66 views

Why does a repulsive force increase the resonance frequency of a resonator?

I have an atomic force microscope with a tuning fork-based sensor. This tuning fork has a probe tip on it that feels a force from the sample that it scans over. Since the tuning fork and probe tip are ...
0 votes
1 answer
45 views

Does a tuning fork's resonance frequency increase with a repulsive force on it in AFM?

I have a tuning fork sensor with a probe tip that would be used in atomic force microscopy. Am I correct that the quartz tuning fork's resonance frequency increases with a repulsive force on it and ...

1
2 3 4 5 6