There are two Friedmann equations, and they are usually written as:
$$ \frac{\dot{a}^2 + kc^2}{a^2} = \frac{8\pi G \rho + \Lambda c^2}{3} \tag{1}$$
$$ \frac{\ddot{a}}{a} = \frac{-4\pi G}{3}\left(\rho + \frac{3p}{c^2}\right) + \frac{\Lambda c^2}{3} \tag{2} $$
The equation you cite is obtained from equation (1) by assuming the cosmological constant, $\Lambda$, is zero. With this assumption the equation simplifies to:
$$ \frac{\dot{a}^2 + kc^2}{a^2} = \frac{8\pi G \rho}{3} \tag{1a}$$
and subtracting $kc^2/a^2$ from both sides gives your equation (I assume that in your equation the $c^2$ has been subsumed into the constant $k$).
$$ \left(\frac{\dot{a}}{a}\right)^2 = \frac{8\pi G \rho}{3} - \frac{kc^2}{a^2} \tag{1b}$$
But then you say:
He said that if the $k$ factor is more negative than the $G$ factor, the universe expansion would have negative deceleration (my emphasis)
But equation (1b) gives us the velocity $\dot{a}$ not the acceleration (or deceleration) $\ddot{a}$. The value of $\dot{a}$ can be positive or negative because when we take the square root of $(\dot{a}/a)^2$ we get both positive or negative roots, but the right side cannot be negative or the square root would be imaginary.
It's the second equation that gives us the acceleration, but the acceleration is independent of $k$ and indeed if we assume $\Lambda = 0$ the acceleration is always negative anyway.
You have not linked the video so I cannot watch it myself to check, but either you have misunderstood what the video is claiming or the video is simply wrong.