I'm begining in Electronic microscopy ( Transmission electronic microscopy and scanning electron microscopy) i was reading an article related to this and i found this information: Since the twisted shape of the trypanosome flagellum (Sherwin and Gull, 1989 ) restricts the use of conventional transmission electron tomography to short portions of the axoneme, we turned to FIB-SEM, which al -lows the collection of hundreds of sequential EM images at 10 nm Z resolution.

please I didn’t unrestand why why Coventional transmission electron tomography is not compatible to otganismes with twisted shapes and what the differences between conventional transmission electron tomography and FIB-SEM

Thank you very much


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