I want to study the optical properties of a molecule under pressure. The method that I am planning to use to apply the pressure on my sample is the following:
A silicone rubber tube is connected to the mouth of a quartz cuvette. The cuvette and the tube are completely filled with the sample which is dissolved in chloroform, and then the tube is capped. The sample is placed in a chamber filled with hexane. Pressure is added into the chamber and transmitted via the hexane, which applies pressure on the sample by compressing the rubber tubing.
This is the diagram:
I want to know what is the amount of pressure that I need to exert on the hexane in order to compress the tubing to say... 10 - 20% of its original volume. And, when I do this, would the pressure inside the cuvette be the same as the pressure outside the cuvette, which I can directly measure with a barometer?
Any information on what type of textbooks would have this information would also be very useful!