My goal is to create an environment of nearly pure nitrogen where only anaerobes can survive. I need to be able to move samples in and out of it. How would you fill it?
You can fill an aquarium with CO2. It is a common demonstration to fill it with smoke or fog and pour CO2 into it. Could you invert the aquarium and fill it with nitrogen?
My thinking is:
- There isn't enough buoyancy for it to rise and displace the oxygen.
- You would only produce an aquarium with a slightly higher percentage of nitrogen.