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Is there any good tutorial on how to read, convert and process .spc files using Python, R, Ruby, Java? Need to extract content of 30 .spc vibrational spectroscopy files to do PCA and descriptive statistics.

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While I haven't used it myself, you could try this here example notebook of a spc package for Python. It seems to work with numpy, so plugging PCA into that via scikit-learn should be easy.

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