In general - No there is not. In the corresponding formalism, the Lagrange or the Hamilton-function will be the starting point to describe the system, and that means that you have to shape the Lagrange function in a suitable way to describe the system. There are no other properties of the system at that point, that have allready been translated into a mathematical object (Like the kinetic Energy, or the Potential energy), you start with the Lagrange function, and in a suitable system you can afterwards identify terms within the Lagrange-function with $V$ or with $T$.
However, choosing a Lagrange-function is not entirely abitrary: Your Lagrange function $L(\vec{x}, \dot{\vec{x}})$ is supposed to describe your system. That means for example, that $L$ has the same symmetries as you observe at your system.
For example, consider the free space: Every region is the same to a flying particle, the laws of physics are the same everywhere. That means your have translational invariance, and hence $L(\vec{x} + \vec{p}, \dot{\vec{x}})=L(\vec{x}, \dot{\vec{x}}) $, for every $\vec{p}$ you can think of. That means $L$ doesn't depend on x. Do the same with Rotational invariance, then you know L can just depend on $|\dot{\vec{x}}|$.
By now L has the form
$$
L= c f(|\dot{\vec{x}}|)
$$
If you demand that every inertial frame of reference will yield the same equations of motion (galileian invariance), then you demand that $$L(\dot{\vec{x}}) = L(\dot{\vec{x}}+\vec{v}) + D(\dot{\vec{x}}) \\
D(\vec{x},\dot{\vec{x}}) = \nabla g(x)\dot{\vec{x}}$$
This is a less rigid condition, the Term $D$ is a gauge-term and doesn't change the equations of motion. One way to satisfy this condition is to choose:
$$
L= c \dot{\vec{x}^2}
$$
Then
$$
L(\dot{\vec{x}}+\vec{v}) = c \dot{\vec{x}^2} + c \vec{v}^2 + 2 c \vec{v} \dot{\vec{x}} = \tilde{L}(\dot{\vec{x}})
$$
$L$ and $\tilde{L}$ will lead to the same equations of motion in two different frames of reference (as you demanded it). L is now shaped in a way that regards homogenity of time, of space, isotropy of space, and free choice of your frame of reference to be symmetries of the system. Now look at the outcome:$$
L= c \dot{\vec{x}^2}
$$
Up to constants (which will come in to play when you define units), you have the kinetic Energy, and you know this function to correctly describe the motion of a free particle. But you did't know in the first place, that there is something like newtons axioms, or something like energy. Everything you knew where the symmetries, and using them, you arrived at this point. In the same way you can demand gauge invariance, then you will arrive at the electromagnetic coupling terms in the Lagrange-function, and so on.