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Jun 15, 2023 at 13:37 comment added CoffeeBiscuit Usually only 1 PSF is used in image formation theory for widefield microscopy since the illumination is constant. However in confocal microscope, the illumination is localized so it is represented by a PSF (which is what almost every single text I read talks about: resolution is not infinite because of diffraction limit). If it helps, we can use the idea used in Image Scanning Microscopy (PRL 104, 198101 (2010)) to think about the PSF. Illumination PSF describes the probability portion of the sample is illuminated while detection PSF is detection probability by detector from a point source.
Jun 15, 2023 at 12:56 comment added garyp The PSFs. Why are two needed, and what do they represent. I suspect my issue is ignorance of the application. I also suspect that I'm not the only ignoramous.
Jun 14, 2023 at 21:40 comment added Kyle Kanos @garyp Are you questioning the abbreviations, which literally follow the full text phrase? Or do you mean the actual PSFs used for each?
Jun 14, 2023 at 14:24 answer added The Photon timeline score: 1
Jun 14, 2023 at 10:58 comment added garyp Can you explain what PSF_ill and PSF_det are? They are not generally known terms, and may be specific to the kind of microscopy you are doing.
Jun 14, 2023 at 10:47 history edited Qmechanic CC BY-SA 4.0
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Jun 14, 2023 at 9:53 history asked CoffeeBiscuit CC BY-SA 4.0