Timeline for Can you use PCR to make a standard kg?
Current License: CC BY-SA 3.0
3 events
| when toggle format | what | by | license | comment | |
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| Jul 18, 2012 at 0:52 | comment | added | Ron Maimon | I checked the Taq polemerase, the errors of 1:9000 are of the type that don't matter for C-G random replication (no AT), the error would be swapping a G for a C, and this error doesn't amplify if the bases are made correct at the end. The issue is whether you can assure there are no G-G pairs of C-C pairs at the end. | |
| Jul 17, 2012 at 16:47 | comment | added | Ron Maimon | YOu don't need isotopic purity, and if you have a C-G strand, some errors don't matter. If you have an inserted base, that matters, but I don't think that happens even 1 in a billion billion, it makes the two strands mismatch in number. THe error is for complementary codon copying, and this fixes itself in a pure CG enviroment. | |
| Jul 17, 2012 at 15:56 | history | answered | John Rennie | CC BY-SA 3.0 |