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Jun
12
comment Absorption Spectral Broadening
I agree that the broadening is inhomogenous because we are observing Gaussian peaks. Could you recommend a specific text, article, or site where I could find out more about these solvent interactions? Most of the papers I've read attribute the Gaussian line shape to dipole coupling in exciton transfer systems, and so I'd like to examine these other, faster interactions you describe. Thanks very much!
Jun
10
comment Absorption Spectral Broadening
This got me thinking- I'm dealing primarily with an RET between chromophores in a biological system, and so I've been assuming that effects due to motion in liquid are second order compared to perturbations and broadening effects due to the actual coupling and energy distribution within the system. Is this a faulty assumption? I'd be interested in knowing if there's a handy rule--of-thumb for the magnitude of the effects of various broadening mechanisms. thank you!
Jun
10
comment Absorption Spectral Broadening
This makes sense to me thermodynamically. But I know that certain photosynthetic spectra fall prey to things like the Stokes shift, in which thermal energy is lost uniformly between excitation and emission, and so the emission spectrum is redshifted relative to the absorption spectrum. It seems to me like there could be non-uniform phenomena like this effect, which is why I'm curious whether the inverse rule is true in all cases. thanks very much for your answer
Jun
10
comment Absorption Spectral Broadening
I apologize, the question was ambiguous. I'm more interested in the qualitative reasons why absorption peaks are broadened. If you could direct me to a resource on the math, I'd appreciate it, but there's no need to waste your effort writing any of the math up for me. The spectroscopy is being done at a fixed low temperature, and so Doppler effects are likely less important than others. Thanks!
Jun
8
awarded  Student
Jun
8
asked Absorption Spectral Broadening