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16
votes
4answers
22k views

What do ants see?

After watching some ants in my garden today, and then looking at this very illuminating demonstration, I got to wondering, about what they would see. Not specifically ants (I understand their ...
8
votes
2answers
2k views

Which is the smallest known particle that scientists have actually *seen with their eyes*? [closed]

Which is the smallest particle that has been actually seen by the scientists? When I say "actually seen", (may be using some ultra advanced microscope or any other man made eye, using any wavelength ...
8
votes
0answers
333 views

Measurement of Tangential Momentum Accomodation?

(this question is a crosspost from theoretical physics.) I am using atomic force microscopy (AFM) for characterizing pores of the size of nanometers for application in gas flow. For this, knowing ...
6
votes
3answers
390 views

Can we make images of single atoms?

I was wondering how far in imaging physics had gotten. Do we hold the technology to actually take a picture of, say, an alpha particle, or even a single atom? I realise we aren't talking about camera ...
6
votes
2answers
139 views

PSF Measurements In Fluorescence Imaging

Quite a technical question! I have measured the Point Spread Function of 100nm fluorescent breads with my Olympus scanning head. I'm two-photon exciting the beads with a wavelength of 800nm and ...
5
votes
1answer
156 views

Why does the colour of a thing change when under huge magnification?

For instance, this image: shows human eyelashes close up. The lashes look green, in fact the whole surface area has a strange tint of green Why is this?
5
votes
2answers
386 views

Optics alignment of a confocal scanning microscope

I am facing a challenge in my project regarding optical alignment. See the figure: The challenge is with the vertical optical system alignment. I considered placing a mirror and check back if the ...
4
votes
2answers
208 views

How does QM allow imaging of individual electron orbitals?

Question: Why does the uncertainty principle allow probing of characteristics specific to the electron orbital distribution? If you measure an electron's position/momentum, then after you measure ...
4
votes
0answers
32 views

How does a microscopes optics expose defects in the users vision?

I've got cataracts in both eyes. My vision is correctable to 20/30, so the cataracts are essentially a non-issue in daily living. But when I use a microscope, which I do daily, (binocular, zoom ...
3
votes
3answers
254 views

What if $\gamma$-rays in Electron microscope?

I was referring Electron microscopes and read that the electrons have wavelength way less than that of visible light. But, the question I can't find an answer was that, If gamma radiation has the ...
3
votes
1answer
692 views

How does an electron microscope work?

I am a physics novice. Google tells me that electron microscopes work much like their optical counterparts -- but the analogy falls apart for me when I think about what I'm "viewing." Obviously, you ...
2
votes
1answer
330 views

Dark and bright areas around atoms in a scanning tunnelling microscope image

Recently IBM created world’s smallest ever animation on an atomic scale video. Researchers made the animation using a scanning tunnelling microscope to move thousands of carbon monoxide molecules to ...
2
votes
1answer
71 views

What are the theoretical prerequisites for experimental work with SEM and TEM? [closed]

What level of knowledge and in what areas would be required to perform experimental work with an SEM (Scanning Electron Microscope) and a TEM (Transmission Electron Microscope) respectively? E.g. ...
2
votes
0answers
33 views

Infinity Corrected Microscope - Building from Scratch

I took an optics course a few years back, and am trying to figure out how to build an infinity-corrected microscope from discrete optical components which are listed in references [2] (lenses) and [3] ...
1
vote
2answers
93 views

Microscopy types and techniques? [closed]

I didn't get the basic difference in between the different types of microscopy. for example there are several different microscopy techniques are available such as Bright field, Dark field, Confocal, ...
1
vote
2answers
114 views

Can protons in the nucleus of an atom be aligned by electromagnetic fields?

Can protons in the nucleus of an atom be aligned by electromagnetic fields? If so can it be done around $-135°C$ zero?
1
vote
1answer
319 views

Does coverslip thickness matter for high NA oil immersion objectives?

This question is related to Impact of covering glass on lens performance. I use a 63x TIRF objective with a numerical aperture of 1.46 and oil immersion. The immersion oil has an index of ...
1
vote
1answer
129 views

Electric Potential at nano scale

I’ve a got a question; and I am hopeful that you can provide any information or direct me to a better resource. I'm not a physicist; so please correct me if I'm wrong. Scanning Probe Microscopy (SPM) ...
1
vote
1answer
118 views

Calculating the magnification of an optical microscopy system

I have a microscopy system that is set up as follows : DSLR with a crop factor of 1.6x ( equivalent to magnification, I believe ) An adaptor optic, for attach the DSLR to the microscope, listed as a ...
1
vote
0answers
44 views

Improvement of microscope resolution with Oil [closed]

Why and when do we need to place oil over the sample to achieve higher optical resolution ? Is this idea is valid for the enhancement of all optical microscopy techniques and magnification scales ?
1
vote
1answer
60 views

Input parameters for the reconstruction algorithm in Digital Holography

I've been browsing some Digital Holography papers these days, and have come across this fundamental question. When you reconstruct the complex amplitude for the object image, you use e.g. Fresnel ...
1
vote
0answers
74 views

Scaling an electron microscope to fit within 1 cubic cm when running multiple parallel scans [closed]

Yes, I'm serious. What components correspond to an individual scan vrs what components can be cascaded across all objects? Ex: The vacuum chamber can be cascaded ( colocate the sample chambers ...
0
votes
3answers
82 views

Do nanoscopes exist?

We are mostly all familiar with a microscope, and know that it helps to see MICRO components, like stuff that is photolithographically etched on silicon semiconductor die. (The latter can also be ...
0
votes
1answer
58 views

Confocal Microscopy

In the context of Confocal Microscopy literature state, "spatial rejection of out of phase light".Is that mean only light which is pass through the pinhole is used and the rest is blocked ?
0
votes
1answer
44 views

How long does it take to scan a typical scanning electron microscope image?

I suppose the answer depends on resolution and imaging area, but can you provide some ball park measures of imaging times with an SEM?
0
votes
1answer
132 views

Making or Demonstrating Principle of Electron Microscope

is it possible to either demonstrate the principle or make a SEM ( electron microscope ) at home or lab as an enthusiast?? and how can i start?
0
votes
1answer
50 views

Methods for determination of crystal lattice's homogeneity

I know of X-Ray diffraction, which produces a pattern corresponding to the inverse Fourier Transform of the lattice (reciprocal lattice). While this method is widely employed, it provides more ...
0
votes
1answer
32 views

Scanning Electron Microscopy: Explanation of Numerical aperture and Extraction Field

I am trying to understand what numerical aperture and extraction field means in a scanning electron microscope. From what I understand numerical aperture is used to set the Depth of Focus which is ...
0
votes
1answer
72 views

Scanning electron microscope imaging

In a scanning electron microscopy, secondary electrons are defined as the electrons which obey inelastic scattering whereas backscattering electron follow elastic scattering. Now my question ...
0
votes
0answers
21 views

Secondary electron gain vs. secondary electron yield

The context of the question is an electron microscope. Can someone explain me the difference between secondary electron gain and secondary electron yield?
0
votes
0answers
34 views

Interaction of SEM electron beam with material

When an electron incident on a material what is the interaction between electron and material? The question is about SEM (Scanning electron microscope). So, the electrons beam will interact ...
-1
votes
1answer
41 views

Which microscopy technique is for wet condition? [closed]

I am searching for a microscopy technique in wet conditions. It must be possible to see through the specimen. ESEM is good but can't see through. TEM needs high vacuum which will boil away water. The ...