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22 views

Simulating a noisy image: Poisson Noise Clarification

I'm currently working to simulate an image that mirrors the output of a CMOS camera we have in the lab. My images include Poisson (photon) noise as well as a gaussian (readout) noise. My issue is ...
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0answers
51 views

How was microscope-level zoom created by a lens and a water droplet?

It was a rainy night. My glasses were speckled with fresh water droplets. I looked at a distant street light and I was surprised to see cells - a single cell was zoomed in to the level where I could ...
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0answers
32 views

Cheap DIY Zernike-Phasecontrast: Optical Thickness of a inkjet/laser printed contour for phasering? [closed]

Rough guess: Optical Thickness of a inkjet/laser printed contour? Edit I was wondering if there is any research regarding the refractive index and the height of an inkjet or laser printed contour?  ...
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1answer
55 views

What are these microscope circles?

This is a photo I have taken with a microscope (100x objective). What are these circles? When I rotate the eyepiece or the objective they stay still. Are they particles on the mirror? In this case ...
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2answers
857 views

Magnification in compound microscope

I was studying about compound microscope here I don't see why we multiply linear magnification of objective with angular magnification of eyepiece. Shouldn't it be both angular or both linear? Can ...
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1answer
79 views

Is this incredible microscope technology real? [closed]

I recall reading about the relatively old invention of a microscope-like device; apparently able to zoom much further (and with greater clarity) than more "advanced" modern technology. I don't recall ...
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0answers
11 views

Relation between phase of dark field and bright field images

I am trying to understand how the super-resolution technique based on Fourier Ptychography 1. In the paper, we run phase retrieval algorithms using images captured using illumination at different ...
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0answers
48 views

A lot of high power microscopes have 2500x magnification, why that specific number?

I've been shopping around for a microscope, and I've noticed something I didn't really expect. Across different brands, across different times (look at some "vintage stuff"), many many microscopes ...
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0answers
25 views

How is the Rayleigh criterion connected to the Abbe limit

I am interrested whether one can derive a formula for the point resolution (like Abbe did) of an optical system from the Rayleigh criterion (without the use of small angle approximation i.e. $\rm{sin}(...
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1answer
54 views

What is the accuracy when firing an electron at a target?

Consider firing an electron at a target. Let the target be at a distance $d$ and the electron be travelling at a non-relativistic speed $v$. How can we estimate the maximum possible accuracy ...
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0answers
33 views

Quantify shade-off and halo effect in phase contrast microscopy

Shade-off and halo effect introduce distortion to phase contrast images. I am trying to utilize these features to write a program which could identify spherical cells from (positive) phase contrast ...
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0answers
21 views

How to get rid of optical reflections in microscopy?

I am running a Raman microscopy experiment with polished silicon wafers. Each time I make an optical image of the wafer surface, reflections appear to the left of the image. This is even worse for ...
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0answers
22 views

determine axial resolution in microscopy

I want to determine the axial resolution of a fluorescence microscope. As I understand, this is commonly done employing a thin fluorescent specimen and performing an axial scan. The axial resolution ...
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0answers
14 views

Microscopy - Depth of Field Shrinks with Magnification

From empirical results I was able to gather that the DOF of a microscope I was working with had been reduced, when I swapped the tube lens to a one with greater magnification (but the same objective ...
4
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1answer
90 views

Beating the Diffraction Limit with NSOM

I am trying to understand exactly why we can beat the diffraction limit when using near-field scanning optical microscopy (NSOM). For those who aren't familiar with NSOM, check out this article: ...
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0answers
8 views

what are the things that affect the ion mobility of a particular material?

what factors are important in ionic transition in a substance? please use easy word to answer as I am a high school student.
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3answers
124 views

How to measure thickness of an ultra-thin metal layer?

I have a sample of a metal (aluminum/oxide) layer with a gradual thickness ranging from monolayer to 100nm. This layer is deposited on a transparent substrate, like glass. How can I measure this ...
0
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1answer
39 views

How does a high-speed atomic force microscope (HS-AFM) form a realtime image?

Regular low speed AFM uses tapping to image a surface. How does a high-speed AFM (HS-AFM) capable of imaging a whole area in "real time"? (Ando et al., ref) Is it just improved signal processing?
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2answers
42 views

Could neutrinos be used to take high resolution pictures of atomic nuclei?

Of course, this is obviously not feasible with modern technology but is it theoretically possible?
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1answer
61 views

What's the difference between two types of diaphragms?

What's the principal difference between aperture iris diaphragm and field iris diaphragm?
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0answers
29 views

Are the Rayleigh criterion and the Point Spread Function related to each other?

Reading this paper as of now, and at the very end of the appendices, it mentions that: However, assuming a highquality preparation, the limit of resolution for any application is always ...
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1answer
15 views

What is a Light Efficient System?

When reading this paper, I encountered this sentence on the sixth page: When used in conjunction with wide-field microscopy, iterative restoration methods are light efficient. This is most ...
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0answers
65 views

Selecting an epi-illuminated objective for optical microscopy

I am currently trying to improve my silicon microphotography. To provide context: this is what I get with a 10x epi plan objective┬╣: This is what I get with 40x epi plan objectve with NA=0.65: I ...
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1answer
2k views

What's the difference between microscopy and spectroscopy?

Both methods collect particles or electromagnetic waves, and in both methods it's possible to reconstruct a 2D image, which may represent morphology (AFM, LEED for example), electronic structure (STM, ...
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0answers
26 views

What are the figures of merit/ performance measurements I can use for deconvolution?

I'm currently performing deconvolution on a bunch of 2D slices of fluorescent beads using MATLAB. Now I want to measure the performance of different deconvolution methods on the filtering of the PSF(...
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2answers
50 views

Why does resolution decrease due to out of focus blur?

Reading the Wikipedia article on Brightfield Microscopy and I notice the point: 'Low apparent optical resolution due to the blur of out of focus material.' in the Limitations section. I ...
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0answers
22 views

influence of soft iron in magnetic lenses

I'm currently working as an intern in a research group which is specialized in transmission electron microscopes. I'm reading a book from David B. Williams and C. Barry Carter : Transmission electron ...
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1answer
70 views

How to perform a rotation of field of view using only mirrors/lenses?

How does one rotate a "microscope" fluorescence image on the detector using only lenses and mirrors in the set-up shown above? I am trying to do ray tracing to understand how to do this, but lenses ...
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0answers
27 views

How does one go about measuring the Point Spread Function pertaining to a particular microscope?

I am aware of the two ways in which to obtain the PSF: Theoretically and Empirically. I understand that empirically, you would have to use fluorescent beads. However, I am missing information as to ...
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1answer
36 views

Why do fluorescent beads need to be sub resolution to measure the point spread function?

It is mentioned everywhere that fluorescent beads need to be sub resolution for empirically measure the point spread function (PSF). Why is this the case?
1
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1answer
217 views

Stacking lenses for higher magnification (a DIY microscope)

Recently, I have made a DIY microscope stand for my phone according to these specifications. I am using a lens that I found in home, the closest match that I could find is here. I got some great ...
2
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1answer
56 views

Is it possible to view an atom? [duplicate]

Is it possible to view an atom? What would it look like? Would it resemble a traditional atomic diagram?
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2answers
158 views

Software for analysing SEM or TEM images

I am looking for a software to analyse SEM and TEM images of some PLD experiments because I want to characterize the samples, like calculate the concentration of nanoparticles, size, and do some ...
0
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0answers
31 views

Btter using a cheap RGB or Monochrome-LCD as a mask for an incoherent white light source compared to?

To be precise, I'm trying to implement ans adaptive condenser-aperture in a brightfield microscope. It's remote and I don't want to have it mechanical moving all the time when changing the objective ...
2
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1answer
24 views

How can I simulate two fluorophores with a reticle?

Fluorophores typically emit in an incoherent fashion. I thought I could simulate two fluorophores (e.g. single molecule fluors) with a reticle. My concept was to make two small holes in a chrome ...
0
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1answer
33 views

Polarized light microscopy

Why is polarized light used in microscopy for the analysis of rocks(for example)? Why not use unpolarized light? What is it with polarized light that makes the analysis of rocks better? Edit: please ...
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2answers
614 views

How are bond angles determined?

Electron microscopes cannot clearly depict the exact shape and structure of atoms and molecules, even though it does show a vague, cloudy image. In my AP chemistry class, I learned that the bond angle ...
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0answers
50 views

Why the Kondo effect is important for STM?

The Kondo effect is observed when approaching 0K. Why would this effect be important for STM?
10
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2answers
287 views

How do a microscope's optics expose defects in the user's vision?

I've got cataracts in both eyes. My vision is correctable to 20/30, so the cataracts are essentially a non-issue in daily living. But when I use a microscope, which I do daily, (binocular, zoom 7x-30x)...
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0answers
95 views

Why isn't the magnifying power in a compound microscope zero when image is formed at infinity?

I think, in a compound microscope, angular magnification and lateral(or linear) magnification is given by the same formula for both image at infinity and near point(D) And the formula is $$M=m=m_om_e$...
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1answer
93 views

Perfectly flat surface

Looking at microscopic images of work tops I noticed that none are perfectly flat. Is it possible to actually create a perfectly flat surface?
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2answers
121 views

Smallest object resolvable by optical microscopy

I am wondering what is the smallest object you can resolve with an optical microscope. I am aware of the equation $\delta=\frac{\lambda}{2\textrm{NA}}$ that basically gives you the resolution. $\...
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1answer
53 views

Dissolving photoconductor (TiOPc) from Laser Printer drum possible?

When I was thinking of a Lab-On-a Chip Application which combines a lensless microscope and an optical tweezers I saw the ODEP-concept:(http://pubs.rsc.org/en/content/articlelanding/2013/lc/c3lc50351h#...
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0answers
34 views

Interaction with the Specimen in Electron Microscope

I read that Electron microscopes throws a high speed electron beam (in vacuum) on the specimen to create a magnified image of the specimen. I wish to know about the interaction of the electrons of the ...
1
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1answer
159 views

Spherical and chromatic aberration correction

I have some spherical lenses which are 5mm, 1mm and 0,5 mm in diameter, having 100x, 350x and 1000x magnification respectively. While looking at blood samples, I'm having big problems with spherical ...
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0answers
16 views

Could a Kelvin Probe Force Microscope, in principal, be used as a voltmeter?

This question goes into the very nature of the work function that the Kelvin Probe Force Microscope (KPFM) measures. https://en.wikipedia.org/wiki/Kelvin_probe_force_microscope Let's say, you have a ...
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0answers
80 views

What's the difference between “spectromicroscopy” and “microspectroscopy”? [duplicate]

Both definitions that I found are rather vague. (Related question: What's the difference between microscopy and spectroscopy?)
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0answers
23 views

Spherical Aberration Of Electron Lenses

I would like to know why electron lenses can't have negative spherical aberration. Every textbook I have found devotes one or two sentences to saying that Otto Scherzer prooved this 80 years ago, but ...
2
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0answers
86 views

What criterion did Abbe use?

For a microscope (correct me if I am wrong) the Rayleigh Criterion gives us: $$ R=\frac{1.22 \lambda}{NA_{condenser}+NA_{objective}}$$ But with the Abbe diffraction limit: $$ R=\frac{\lambda}{NA_{...
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0answers
50 views

Why don't electron microscopes use huge detectors?

Electron microscopes have limited resolution. I have read experts saying that we need better electron detectors to get better resolution. Why can't we just use bigger detectors? Increase the ...