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1answer
146 views

Making or Demonstrating Principle of Electron Microscope

is it possible to either demonstrate the principle or make a SEM ( electron microscope ) at home or lab as an enthusiast?? and how can i start?
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0answers
383 views

Measurement of Tangential Momentum Accomodation?

(this question is a crosspost from theoretical physics.) I am using atomic force microscopy (AFM) for characterizing pores of the size of nanometers for application in gas flow. For this, knowing ...
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0answers
77 views

Scaling an electron microscope to fit within 1 cubic cm when running multiple parallel scans [closed]

Yes, I'm serious. What components correspond to an individual scan vrs what components can be cascaded across all objects? Ex: The vacuum chamber can be cascaded ( colocate the sample chambers ...
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1answer
407 views

Does coverslip thickness matter for high NA oil immersion objectives?

This question is related to Impact of covering glass on lens performance. I use a 63x TIRF objective with a numerical aperture of 1.46 and oil immersion. The immersion oil has an index of ...
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4answers
31k views

What do ants see?

After watching some ants in my garden today, and then looking at this very illuminating demonstration, I got to wondering, about what they would see. Not specifically ants (I understand their ...
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1answer
825 views

How does an electron microscope work?

I am a physics novice. Google tells me that electron microscopes work much like their optical counterparts -- but the analogy falls apart for me when I think about what I'm "viewing." Obviously, you ...
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2answers
174 views

PSF Measurements In Fluorescence Imaging

Quite a technical question! I have measured the Point Spread Function of 100nm fluorescent breads with my Olympus scanning head. I'm two-photon exciting the beads with a wavelength of 800nm and ...