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1answer
102 views

Methods for determination of crystal lattice's homogeneity

I know of X-Ray diffraction, which produces a pattern corresponding to the inverse Fourier Transform of the lattice (reciprocal lattice). While this method is widely employed, it provides more ...
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1answer
218 views

Scanning electron microscope imaging

In a scanning electron microscopy, secondary electrons are defined as the electrons which obey inelastic scattering whereas backscattering electron follow elastic scattering. Now my question ...
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1answer
139 views

Electric Potential at nano scale

I’ve a got a question; and I am hopeful that you can provide any information or direct me to a better resource. I'm not a physicist; so please correct me if I'm wrong. Scanning Probe Microscopy (SPM) ...
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1answer
223 views

Calculating the magnification of an optical microscopy system

I have a microscopy system that is set up as follows : DSLR with a crop factor of 1.6x ( equivalent to magnification, I believe ) An adaptor optic, for attach the DSLR to the microscope, listed as a ...
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1answer
95 views

What are the theoretical prerequisites for experimental work with SEM and TEM? [closed]

What level of knowledge and in what areas would be required to perform experimental work with an SEM (Scanning Electron Microscope) and a TEM (Transmission Electron Microscope) respectively? E.g. ...
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2answers
905 views

Optics alignment of a confocal scanning microscope

I am facing a challenge in my project regarding optical alignment. See the figure: The challenge is with the vertical optical system alignment. I considered placing a mirror and check back if the ...
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1answer
1k views

Dark and bright areas around atoms in a scanning tunnelling microscope image

Recently IBM created world’s smallest ever animation on an atomic scale video. Researchers made the animation using a scanning tunnelling microscope to move thousands of carbon monoxide molecules to ...
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3answers
996 views

Can we make images of single atoms?

I was wondering how far in imaging physics had gotten. Do we hold the technology to actually take a picture of, say, an alpha particle, or even a single atom? I realise we aren't talking about camera ...
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3answers
389 views

What if $\gamma$-rays in Electron microscope?

I was referring Electron microscopes and read that the electrons have wavelength way less than that of visible light. But, the question I can't find an answer was that, If gamma radiation has the ...
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2answers
236 views

How does QM allow imaging of individual electron orbitals?

Question: Why does the uncertainty principle allow probing of characteristics specific to the electron orbital distribution? If you measure an electron's position/momentum, then after you measure ...
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1answer
157 views

Making or Demonstrating Principle of Electron Microscope

is it possible to either demonstrate the principle or make a SEM ( electron microscope ) at home or lab as an enthusiast?? and how can i start?
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0answers
77 views

Scaling an electron microscope to fit within 1 cubic cm when running multiple parallel scans [closed]

Yes, I'm serious. What components correspond to an individual scan vrs what components can be cascaded across all objects? Ex: The vacuum chamber can be cascaded ( colocate the sample chambers ...
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1answer
431 views

Does coverslip thickness matter for high NA oil immersion objectives?

This question is related to Impact of covering glass on lens performance. I use a 63x TIRF objective with a numerical aperture of 1.46 and oil immersion. The immersion oil has an index of ...
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4answers
33k views

What do ants see?

After watching some ants in my garden today, and then looking at this very illuminating demonstration, I got to wondering, about what they would see. Not specifically ants (I understand their ...
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1answer
858 views

How does an electron microscope work?

I am a physics novice. Google tells me that electron microscopes work much like their optical counterparts -- but the analogy falls apart for me when I think about what I'm "viewing." Obviously, you ...
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2answers
188 views

PSF Measurements In Fluorescence Imaging

Quite a technical question! I have measured the Point Spread Function of 100nm fluorescent breads with my Olympus scanning head. I'm two-photon exciting the beads with a wavelength of 800nm and ...