3
votes
2answers
61 views

Can there be a string so thin as to be invisible, but that can still support a visible weight?

A spider web thread is very thin, yet it can support a spider. Given that the human eye visual acuity is finite, are there materials, natural or man-made, sufficiently thin that a string made from ...
0
votes
0answers
36 views

Microscope Objective / NA, F/#, Exit aperture - when are they non-intuitive?

Numerical aperture (NA) $= sin(\theta)$ where $\theta$ is the half-angle (see: http://en.wikipedia.org/wiki/Numerical_aperture) F/# $= \frac{f}{D}$ (same reference as above) where F/# is the ...
4
votes
0answers
41 views

How does a microscopes optics expose defects in the users vision?

I've got cataracts in both eyes. My vision is correctable to 20/30, so the cataracts are essentially a non-issue in daily living. But when I use a microscope, which I do daily, (binocular, zoom ...
2
votes
1answer
115 views

Infinity Corrected Microscope - Building from Scratch

I took an optics course a few years back, and am trying to figure out how to build an infinity-corrected microscope from discrete optical components which are listed in references [2] (lenses) and [3] ...
0
votes
1answer
95 views

Confocal Microscopy

In the context of Confocal Microscopy literature state, "spatial rejection of out of phase light".Is that mean only light which is pass through the pinhole is used and the rest is blocked ?
5
votes
1answer
164 views

Why does the colour of a thing change when under huge magnification?

For instance, this image: shows human eyelashes close up. The lashes look green, in fact the whole surface area has a strange tint of green Why is this?
-1
votes
1answer
41 views

Which microscopy technique is for wet condition? [closed]

I am searching for a microscopy technique in wet conditions. It must be possible to see through the specimen. ESEM is good but can't see through. TEM needs high vacuum which will boil away water. The ...
1
vote
0answers
71 views

Improvement of microscope resolution with Oil [closed]

Why and when do we need to place oil over the sample to achieve higher optical resolution ? Is this idea is valid for the enhancement of all optical microscopy techniques and magnification scales ?
1
vote
2answers
121 views

Microscopy types and techniques? [closed]

I didn't get the basic difference in between the different types of microscopy. for example there are several different microscopy techniques are available such as Bright field, Dark field, Confocal, ...
1
vote
1answer
82 views

Input parameters for the reconstruction algorithm in Digital Holography

I've been browsing some Digital Holography papers these days, and have come across this fundamental question. When you reconstruct the complex amplitude for the object image, you use e.g. Fresnel ...
1
vote
1answer
143 views

Calculating the magnification of an optical microscopy system

I have a microscopy system that is set up as follows : DSLR with a crop factor of 1.6x ( equivalent to magnification, I believe ) An adaptor optic, for attach the DSLR to the microscope, listed as a ...
5
votes
2answers
539 views

Optics alignment of a confocal scanning microscope

I am facing a challenge in my project regarding optical alignment. See the figure: The challenge is with the vertical optical system alignment. I considered placing a mirror and check back if the ...
1
vote
0answers
75 views

Scaling an electron microscope to fit within 1 cubic cm when running multiple parallel scans [closed]

Yes, I'm serious. What components correspond to an individual scan vrs what components can be cascaded across all objects? Ex: The vacuum chamber can be cascaded ( colocate the sample chambers ...
1
vote
1answer
350 views

Does coverslip thickness matter for high NA oil immersion objectives?

This question is related to Impact of covering glass on lens performance. I use a 63x TIRF objective with a numerical aperture of 1.46 and oil immersion. The immersion oil has an index of ...