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17 views

Are the Rayleigh criterion and the Point Spread Function related to each other?

Reading this paper as of now, and at the very end of the appendices, it mentions that: However, assuming a highquality preparation, the limit of resolution for any application is always ...
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1answer
12 views

What is a Light Efficient System?

When reading this paper, I encountered this sentence on the sixth page: When used in conjunction with wide-field microscopy, iterative restoration methods are light efficient. This is most ...
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0answers
22 views

What are the figures of merit/ performance measurements I can use for deconvolution?

I'm currently performing deconvolution on a bunch of 2D slices of fluorescent beads using MATLAB. Now I want to measure the performance of different deconvolution methods on the filtering of the ...
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2answers
36 views

Why does resolution decrease due to out of focus blur?

Reading the Wikipedia article on Brightfield Microscopy and I notice the point: 'Low apparent optical resolution due to the blur of out of focus material.' in the Limitations section. I ...
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0answers
19 views

influence of soft iron in magnetic lenses

I'm currently working as an intern in a research group which is specialized in transmission electron microscopes. I'm reading a book from David B. Williams and C. Barry Carter : Transmission electron ...
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1answer
42 views

How to perform a rotation of field of view using only mirrors/lenses?

How does one rotate a "microscope" fluorescence image on the detector using only lenses and mirrors in the set-up shown above? I am trying to do ray tracing to understand how to do this, but lenses ...
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0answers
21 views

How does one go about measuring the Point Spread Function pertaining to a particular microscope?

I am aware of the two ways in which to obtain the PSF: Theoretically and Empirically. I understand that empirically, you would have to use fluorescent beads. However, I am missing information as to ...
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1answer
31 views

Why do fluorescent beads need to be sub resolution to measure the point spread function?

It is mentioned everywhere that fluorescent beads need to be sub resolution for empirically measure the point spread function (PSF). Why is this the case?
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1answer
48 views

Stacking lenses for higher magnification (a DIY microscope)

Recently, I have made a DIY microscope stand for my phone according to these specifications. I am using a lens that I found in home, the closest match that I could find is here. I got some great ...
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1answer
49 views

Is it possible to view an atom? [duplicate]

Is it possible to view an atom? What would it look like? Would it resemble a traditional atomic diagram?
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0answers
22 views

Btter using a cheap RGB or Monochrome-LCD as a mask for an incoherent white light source compared to?

To be precise, I'm trying to implement ans adaptive condenser-aperture in a brightfield microscope. It's remote and I don't want to have it mechanical moving all the time when changing the objective ...
2
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1answer
22 views

How can I simulate two fluorophores with a reticle?

Fluorophores typically emit in an incoherent fashion. I thought I could simulate two fluorophores (e.g. single molecule fluors) with a reticle. My concept was to make two small holes in a chrome ...
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1answer
20 views

Polarized light microscopy

Why is polarized light used in microscopy for the analysis of rocks(for example)? Why not use unpolarized light? What is it with polarized light that makes the analysis of rocks better? Edit: please ...
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2answers
122 views

How are bond angles determined?

Electron microscopes cannot clearly depict the exact shape and structure of atoms and molecules, even though it does show a vague, cloudy image. In my AP chemistry class, I learned that the bond angle ...
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0answers
31 views

Why the Kondo effect is important for STM?

The Kondo effect is observed when approaching 0K. Why would this effect be important for STM?
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3answers
67 views

How to measure thickness of an ultra-thin metal layer?

I have a sample of a metal (aluminum/oxide) layer with a gradual thickness ranging from monolayer to 100nm. This layer is deposited on a transparent substrate, like glass. How can I measure this ...
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0answers
56 views

Why isn't the magnifying power in a compound microscope zero when image is formed at infinity?

I think, in a compound microscope, angular magnification and lateral(or linear) magnification is given by the same formula for both image at infinity and near point(D) And the formula is ...
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0answers
47 views

Selecting an epi-illuminated objective for optical microscopy

I am currently trying to improve my silicon microphotography. To provide context: this is what I get with a 10x epi plan objective¹: This is what I get with 40x epi plan objectve with NA=0.65: I ...
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1answer
58 views

Perfectly flat surface

Looking at microscopic images of work tops I noticed that none are perfectly flat. Is it possible to actually create a perfectly flat surface?
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2answers
76 views

Smallest object resolvable by optical microscopy

I am wondering what is the smallest object you can resolve with an optical microscope. I am aware of the equation $\delta=\frac{\lambda}{2\textrm{NA}}$ that basically gives you the resolution. ...
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0answers
32 views

Interaction with the Specimen in Electron Microscope

I read that Electron microscopes throws a high speed electron beam (in vacuum) on the specimen to create a magnified image of the specimen. I wish to know about the interaction of the electrons of the ...
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1answer
42 views

Dissolving photoconductor (TiOPc) from Laser Printer drum possible?

When I was thinking of a Lab-On-a Chip Application which combines a lensless microscope and an optical tweezers I saw the ...
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0answers
13 views

Could a Kelvin Probe Force Microscope, in principal, be used as a voltmeter?

This question goes into the very nature of the work function that the Kelvin Probe Force Microscope (KPFM) measures. https://en.wikipedia.org/wiki/Kelvin_probe_force_microscope Let's say, you have a ...
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1answer
98 views

Spherical and chromatic aberration correction

I have some spherical lenses which are 5mm, 1mm and 0,5 mm in diameter, having 100x, 350x and 1000x magnification respectively. While looking at blood samples, I'm having big problems with spherical ...
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0answers
38 views

What's the difference between two types of diaphragms?

What's the principal difference between aperture iris diaphragm and field iris diaphragm?
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0answers
22 views

Spherical Aberration Of Electron Lenses

I would like to know why electron lenses can't have negative spherical aberration. Every textbook I have found devotes one or two sentences to saying that Otto Scherzer prooved this 80 years ago, but ...
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0answers
49 views

Why don't electron microscopes use huge detectors?

Electron microscopes have limited resolution. I have read experts saying that we need better electron detectors to get better resolution. Why can't we just use bigger detectors? Increase the ...
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0answers
34 views

Magnification: Microscopes Telescopes

What is the difference between the way a microscope magnifies say a star and a telescope say a cell? Why is it, that the eyepiece of a telescope magnifies stronger when it is smaller and with a ...
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1answer
30 views

To describe crystallity structures of this ferromagnetic material

MOKE microscope picture of the ferromagnetic Material $Co_{40} Fe_{40} B_{20}$ of 20 nm thin film All other pictures look the same, also from different angles: [0,360] by 15 degree separation. I ...
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0answers
60 views

Importance of thickness of SiO2-substrate for observing graphene's monolayer

I've discovered that one should use 300-nm-thick SiO2 substrate in order to effectively observe graphene's monolayer through optical microscope. If thickness differs even by 5%, i.e. 315 nm, then ...
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0answers
145 views

Why do spatial filters use microscope objectives, and not other types of lenses?

A spatial filter is a device to 'clean up' a laser beam with an irregular intensity profile, and create a smooth Gaussian profile at the output. It is usually said (e.g. here) that you need a ...
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1answer
57 views

What is the importance of reciprocal lattice?

Reciprocal lattice is the diffraction plot of a crystal. Now with the STM instrument we can get the get the topology of the crystal, so what is the importance of reciprocal lattice or the Brillouin ...
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1answer
26 views

What is bias voltage and what is it's function in a Scan Tunneling Microscope?

I was reading about the Scan Tunneling Microscope and I read that a 'bias voltage' is needed to realize a current of electrons between the needle and the sample. I don't know what the definition of ...
1
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1answer
71 views

Why subwavelength objects can not be seen with optical microscope?

What would happen if we would take a very small sphere around 200nm diameter and try to detect it from the most efficient optical microscope? Technically, the Rayleigh diffraction limit prevents the ...
2
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0answers
72 views

What criterion did Abbe use?

For a microscope (correct me if I am wrong) the Rayleigh Criterion gives us: $$ R=\frac{1.22 \lambda}{NA_{condenser}+NA_{objective}}$$ But with the Abbe diffraction limit: $$ ...
3
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1answer
61 views

What are these wavish patterns around atoms on microscopic images?

This is an IBM movie about how they move single atoms: http://www.youtube.com/watch?v=oSCX78-8-q0 There are often some "wavish" pattern around atoms on these pictures, like below some of them marked ...
3
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0answers
60 views

Beating the Diffraction Limit with NSOM

I am trying to understand exactly why we can beat the diffraction limit when using near-field scanning optical microscopy (NSOM). For those who aren't familiar with NSOM, check out this article: ...
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2answers
266 views

Resolving power of a microscope?

I was reading up on the resolution of a microscope. I read (in some lecture notes) that the size of the limiting spot size is $1.22 \lambda f/W$. But that the smallest resolvable feature has a size ...
0
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1answer
57 views

How does an electron beam condenser work?

For example, a scanning electron microscope has multiple condensers that "focus" the beam into a smaller spot size. How does a condenser actually change the direction of electron flow in a non-uniform ...
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1answer
52 views

Why is there no fluorescence background in CARS?

In CARS (coherent anti-Stokes Raman scattering), the detected signal is blue-shifted with respect to the excitation - how does this mean that there is no fluorescence or that the fluorescence can be ...
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0answers
48 views

Microscopy: aperture matching and Huygen's principle

I have repeatedly read that the maximum spatial resolution of a microscope occurs when the aperture of the objective and condenser are the same. However, I don't understand why. The resolution is ...
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0answers
17 views

A question about elastically scattering

In an electron diffraction pattern, the elastically scattered electrons are distributed over distances in the pattern corresponding to resolutions considerably higher than 1 Å. My question is can it ...
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0answers
24 views

A problem about Fresnel fringes of electron microscopy

My question is simple: Why do the Fresnel fringes at the edge of a hole in a reticulated carbon film become larger and more widely spaced when the defocus is increased? This occurs whether you are ...
1
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1answer
875 views

What's the difference between microscopy and spectroscopy?

Both methods collect particles or electromagnetic waves, and in both methods it's possible to reconstruct a 2D image, which may represent morphology (AFM, LEED for example), electronic structure (STM, ...
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0answers
79 views

What's the difference between “spectromicroscopy” and “microspectroscopy”? [duplicate]

Both definitions that I found are rather vague. (Related question: What's the difference between microscopy and spectroscopy?)
3
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5answers
403 views

Isn't all light polarised?

I apologize if my question does not make sense.(I'm teaching myself microscopy.) So reading Fundamentals of Light Microscopy and electronic imaging by Douglas&Murphy, at one point the author ...
0
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1answer
74 views

Resolution of experiment is lower than the detector, so how to weigh the data?

I am attempting to create an atomic model based on data from a transmission electron microscope (TEM). Basically you shoot electrons at bunch of identical molecules stuck to a grid, and look at the ...
7
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1answer
153 views

Has the Nobel committee mixed up this years prizes for Physics and Chemistry? [closed]

The title of the question is tongue-in-cheek but the question remains: How does the Nobel committee delineate the fields when awarding work which is of such an inter-disciplinary nature. The chemistry ...
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2answers
132 views

Software for analysing SEM or TEM images

I am looking for a software to analyse SEM and TEM images of some PLD experiments because I want to characterize the samples, like calculate the concentration of nanoparticles, size, and do some ...
4
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2answers
163 views

Can there be a string so thin as to be invisible, but that can still support a visible weight?

A spider web thread is very thin, yet it can support a spider. Given that the human eye visual acuity is finite, are there materials, natural or man-made, sufficiently thin that a string made from ...