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Double slit experiment and perforated walls' properties

I have a doubt about how double slit experiment is made. Let's think about the perforated wall, what are the requirement for it? Can a photographic plate could be used as a wall ? I see a problem ...
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What's inside the slit in double slit experiment?

If double slit experiment is done in a environment with air, then slits could also contain air made up of (approx. 80% nitrogen, 20% oxygen), then there is not empty space inside the slit. How can it ...